研究業績
2025年度
Kouga T, Miwa T, Wei FY, Sunami K, Tomizawa K.
Mitochonic acid 5 mitigates age-related hearing loss progression by targeting defective 2-methylthiolation in mitochondrial transfer RNAs
Front Cell Neurosci. 2025 Apr 7;19:1541347.
Morishima T, Fakruddin M, Kanamori Y, Masuda T, Ogawa A, Wang Y, Schoonenberg VAC, Butter F, Arima Y, Akaike T, Moroishi T, Tomizawa K, Suda T, Wei FY, Takizawa H.
Mitochondrial translation regulates terminal erythroid differentiation by maintaining iron homeostasis
Sci Adv. 2025 Feb 21;11(8):eadu3011.
Tejima M, Hashimoto T, Ohno O, Hoshina T, Takasaki K, Taniguchi S, Nakamura K, Wei FY, Tomizawa K, Matsuno K.
Eperisone Analogs, Rescuers of MiaB Defects As a Prokaryotic Homologue of CDKAL1, Suppress Blood Glucose Elevation in Rats
ACS Med Chem Lett. 2025 Jan 17;16(2):311-316.
Matsuda S, Nakayama M, Do Y, Ishiuchi T, Yagi M, Wanrooij S, Nakada K, Wei FY, Ichiyanagi K, Sasaki H, Kang D, Yasukawa T.
TEFM facilitates transition from RNA synthesis to DNA synthesis at H-strand replication origin of mtDNA
Commun Biol. 2025 Feb 8;8(1):202.
Arai K, Ono Y, Hirai N, Sugiura Y, Kaneko K, Matsuda S, Iio K, Kajino K, Saitoh T, Wei FY, Katagiri H, Inoue A.
Chemogenetic activation of hepatic G12 signaling ameliorates hepatic steatosis and obesity
Biochim Biophys Acta Mol Basis Dis. 2025 Feb;1871(2):167566.
2024年度
Oshima HS, Ogawa A, Sano FK, Akasaka H, Kawakami T, Iwama A, Okamoto HH, Nagiri C, Wei FY, Shihoya W, Nureki O.
Structural insights into the agonist selectivity of the adenosine A3 receptor
Nat Commun. 2024 Nov 7;15(1):9294.
Matsuura J, Akichika S, Wei FY, Suzuki T, Yamamoto T, Watanabe Y, Valášek LS, Mukasa A, Tomizawa K, Chujo T.
Human DUS1L catalyzes dihydrouridine modification at tRNA positions 16/17, and DUS1L overexpression perturbs translation
Commun Biol. 2024 Oct 2;7(1):1238.
Ishikawa K, Miyata D, Hattori S, Tani H, Kuriyama T, Wei FY, Miyakawa T, Nakada K.
Accumulation of mitochondrial DNA with a point mutation in tRNALeu(UUR) gene induces brain dysfunction in mice
Pharmacol Res. 2024 Oct;208:107374.
Tresky R, Miyamoto Y, Nagayoshi Y, Yabuki Y, Araki K, Takahashi Y, Komohara Y, Ge H, Nishiguchi K, Fukuda T, Kaneko H, Maeda N, Matsuura J, Iwasaki S, Sakakida K, Shioda N, Wei FY, Tomizawa K, Chujo T.
TRMT10A dysfunction perturbs codon translation of initiator methionine and glutamine and impairs brain functions in mice
Nucleic Acids Res. 2024 Aug 27;52(15):9230-9246.
Ahmad RNR, Zhang LT, Morita R, Tani H, Wu Y, Chujo T, Ogawa A, Harada R, Shigeta Y, Tomizawa K, Wei FY.
Pathological mutations promote proteolysis of mitochondrial tRNA-specific 2-thiouridylase 1 (MTU1) via mitochondrial caseinolytic peptidase (CLPP)
Nucleic Acids Res. 2024 Feb 9;52(3):1341-1358.
2023年度
Barayeu U, Sawa T, Nishida M, Wei FY, Motohashi H, Akaike T.
Supersulfide biology and translational medicine for disease control
Br J Pharmacol. 2023 Oct 23. Review.
Matsunaga T, Sano H, Takita K, Morita M, Yamanaka S, Ichikawa T, Numakura T, Ida T, Jung M, Ogata S, Yoon S, Fujino N, Kyogoku Y, Sasaki Y, Koarai A, Tamada T, Toyama A, Nakabayashi T, Kageyama L, Kyuwa S, Inaba K, Watanabe S, Nagy P, Sawa T, Oshiumi H, Ichinose M, Yamada M, Sugiura H, Wei FY, Motohashi H, Akaike T.
Supersulphides provide airway protection in viral and chronic lung diseases
Nat Commun. 2023 Jul 25;14(1):4476.
Miwa T, Katsuno T, Wei FY, Tomizawa K.
Mitochondrial alterations in the cochlea of Cdk5rap1-knockout mice with age-related hearing loss
FEBS Open Bio. 2023 Jul;13(7):1365-1374.
Ogawa A, Ohira S, Kato Y, Ikuta T, Yanagida S, Mi X, Ishii Y, Kanda Y, Nishida M, Inoue A, Wei FY.
Activation of the urotensin-II receptor by remdesivir induces cardiomyocyte dysfunction
Commun Biol. 2023 May 12;6(1):511.
Matsumura Y, Wei FY, Sakai J.
Epitranscriptomics in metabolic disease
Nat Metab. 2023 Mar;5(3):370-384. Review.
Murakami Y, Wei FY, Kawamura Y, Horiguchi H, Kadomatsu T, Miyata K, Miura K, Oike Y, Ando Y, Ueda M, Tomizawa K, Chujo T.
NSUN3-mediated mitochondrial tRNA 5-formylcytidine modification is essential for embryonic development and respiratory complexes in mice
Commun Biol. 2023 Mar 22;6(1):307.
2022年度
Nagayoshi Y, Nishiguchi K, Yamamura R, Chujo T, Oshiumi H, Nagata H, Kaneko H, Yamamoto K, Nakata H, Sakakida K, Kunisawa A, Adachi M, Kakizoe Y, Mizobe T, Kuratsu JI, Shimada S, Nakamori Y, Matsuoka M, Mukoyama M, Wei FY, Tomizawa K.
t6A and ms2t6A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity.
Biomolecules. 2022 Sep 3;12(9):1233.
Tani H, Ishikawa K, Tamashiro H, Ogasawara E, Yasukawa T, Matsuda S, Shimizu A, Kang D, Hayashi JI, Wei FY, Nakada K.
Aberrant RNA processing contributes to the pathogenesis of mitochondrial diseases in trans-mitochondrial mouse model carrying mitochondrial tRNALeu(UUR) with a pathogenic A2748G mutation
Nucleic Acids Res. 2022 Sep 9;50(16):9382-9396.
Yakita M, Chujo T, Wei FY, Hirayama M, Kato K, Takahashi N, Naganuma K, Nagata M, Kawahara K, Nakayama H, Tomizawa K.
Extracellular N6-isopentenyladenosine (i6A) addition induces cotranscriptional i 6A incorporation into ribosomal RNAs
RNA. 2022 Jul;28(7):1013-1027.
Monde K, Satou Y, Goto M, Uchiyama Y, Ito J, Kaitsuka T, Terasawa H, Monde N, Yamaga S, Matsusako T, Wei FY, Inoue I, Tomizawa K, Ono A, Era T, Sawa T, Maeda Y.
Movements of Ancient Human Endogenous Retroviruses Detected in SOX2-Expressing Cells
J Virol. 2022 May 11;96(9):e0035622.
Tsutsuki H, Zhang T, Yahiro K, Ono K, Fujiwara Y, Iyoda S, Wei FY, Monde K, Seto K, Ohnishi M, Oshiumi H, Akaike T, Sawa T.
Subtilase cytotoxin from Shiga-toxigenic Escherichia coli impairs the inflammasome and exacerbates enteropathogenic bacterial infection
iScience. 2022 Mar 10;25(4):104050.
[pubmed]
Inatomi T, Matsuda S, Ishiuchi T, Do Y, Nakayama M, Abe S, Kasho K, Wanrooij S, Nakada K, Ichiyanagi K, Sasaki H, Yasukawa T, Kang D.
TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication
Biochim Biophys Acta Mol Cell Res. 2022 Jan;1869(1):119167.
[pubmed]
2021年度
Fukuda H, Chujo T, Wei FY, Shi SL, Hirayama M, Kaitsuka T, Yamamoto T, Oshiumi H, Tomizawa K.
Cooperative methylation of human tRNA3Lys at positions A58 and U54 drives the early and late steps of HIV-1 replication
Nucleic Acids Res. 2021 Nov 18;49(20):11855-11867.
[pubmed]
Ogawa A, Wei FY.
Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
STAR Protoc. 2021 Sep 27;2(4):100848.
[pubmed]
Shi SL, Fukuda H, Chujo T, Kouwaki T, Oshiumi H, Tomizawa K, Wei FY.
Export of RNA-derived modified nucleosides by equilibrative nucleoside transporters defines the magnitude of autophagy response and Zika virus replication
RNA Biol. 2021 Oct 15;18(sup1):478-495.
[pubmed]
Miwa T, Wei FY, Tomizawa K.
Cdk5 regulatory subunit-associated protein 1 knockout mice show hearing loss phenotypically similar to age-related hearing loss
Mol Brain. 2021 May 17;14(1):82.
[pubmed]
Nagayoshi Y, Chujo T, Hirata S, Nakatsuka H, Chen CW, Takakura M, Miyauchi K, Ikeuchi Y, Carlyle BC, Kitchen RR, Suzuki T, Katsuoka F, Yamamoto M, Goto Y, Tanaka M, Natsume K, Nairn AC, Suzuki T, Tomizawa K, Wei FY.
Loss of Ftsj1 perturbs codon-specific translation efficiency in the brain and is associated with X-linked intellectual disability
Sci Adv. 2021 Mar 26;7(13):eabf3072.
[pubmed]
Ogawa A, Nagiri C, Shihoya W, Inoue A, Kawakami K, Hiratsuka S, Aoki J, Ito Y, Suzuki T, Suzuki T, Inoue T, Nureki O, Tanihara H, Tomizawa K, Wei FY.
N 6-methyladenosine (m 6 A) is an endogenous A3 adenosine receptor ligand
Mol Cell. 2021 Feb 18;81(4):659-674.e7.
[pubmed]
selected as a 'Research Highlight' in Nature Chemical Biology
2020年度
Tomizawa K, Wei FY.
Posttranscriptional modifications in mitochondrial tRNA and its implication in mitochondrial translation and disease
J Biochem. 2020 Nov 1;168(5):435-444. Review
[pubmed]
Kanno A, Asahara SI, Furubayashi A, Masuda K, Yoshitomi R, Suzuki E, Takai T, Kimura-Koyanagi M, Matsuda T, Bartolome A, Hirota Y, Yokoi N, Inaba Y, Inoue H, Matsumoto M, Inoue K, Abe T, Wei FY, Tomizawa K, Ogawa W, Seino S, Kasuga M, Kido Y.
GCN2 regulates pancreatic β cell mass by sensing intracellular amino acid levels
JCI Insight. 2020 May 7;5(9):e128820.
[pubmed]
Hirayama M, Wei FY, Chujo T, Oki S, Yakita M, Kobayashi D, Araki N, Takahashi N, Yoshida R, Nakayama H, Tomizawa K.
FTO Demethylates Cyclin D1 mRNA and Controls Cell-Cycle Progression
Cell Rep. 2020 Apr 7;31(1):107464.
[pubmed]
Miyazaki T, Sasaki SI, Toyoda A, Wei FY, Shirai M, Morishita Y, Ikegami T, Tomizawa K, Honda A.
Impaired bile acid metabolism with defectives of mitochondrial-tRNA taurine modification and bile acid taurine conjugation in the taurine depleted cats
Sci Rep. 2020 Mar 18;10(1):4915.
[pubmed]
2019年度以前
Naito T, Ercan B, Krshnan L, Triebl A, Koh DHZ, Wei FY, Tomizawa K, Torta FT, Wenk MR, Saheki Y.
Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex
Elife. 2019 Nov 14;8:e51401.
[pubmed]
Yamamoto T, Fujimura A, Wei FY, Shinojima N, Kuroda JI, Mukasa A, Tomizawa K.
2-Methylthio Conversion of N6-Isopentenyladenosine in Mitochondrial tRNAs by CDK5RAP1 Promotes the Maintenance of Glioma-Initiating Cells
iScience. 2019 Nov 22;21:42-56.
[pubmed]
Shinoda S, Kitagawa S, Nakagawa S, Wei FY, Tomizawa K, Araki K, Araki M, Suzuki T, Suzuki T.
Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs
Nucleic Acids Res. 2019 Sep 19;47(16):8734-8745.
[pubmed]
Takesue Y, Wei FY, Fukuda H, Tanoue Y, Yamamoto T, Chujo T, Shinojima N, Yano S, Morioka M, Mukasa A, Kuratsu J, Tomizawa K.
Regulation of growth hormone biosynthesis by Cdk5 regulatory subunit associated protein 1-like 1 (CDKAL1) in pituitary adenomas
Endocr J. 2019 Sep 28;66(9):807-816.
[pubmed]
Takenouchi T, Wei FY, Suzuki H, Uehara T, Takahashi T, Okazaki Y, Kosaki K, Tomizawa K.
Noninvasive diagnosis of TRIT1-related mitochondrial disorder by measuring i 6 A37 and ms 2i 6 A37 modifications in tRNAs from blood and urine samples
Am J Med Genet A. 2019 Aug;179(8):1609-1614. Review
[pubmed]
Doki T, Yamashita S, Wei FY, Hara K, Yamamoto T, Zhang Z, Zhang X, Tawara N, Hino H, Uyama E, Kurashige T, Maruyama H, Tomizawa K, Ando Y.
Mitochondrial localization of PABPN1 in oculopharyngeal muscular dystrophy
Lab Invest. 2019 Nov;99(11):1728-1740.
[pubmed]
Mon EE, Wei FY, Ahmad RNR, Yamamoto T, Moroishi T, Tomizawa K.
Regulation of mitochondrial iron homeostasis by sideroflexin 2
J Physiol Sci. 2019 Mar;69(2):359-373.
[pubmed]
Fakruddin, M., Wei, F.-Y., Suzuki, T., Asano, K., Kaieda, T., Omori, A., Izumi, R., Fujimura, A., Kaitsuka, T., Miyata, K., Araki, K., Oike, Y., Scorrano, L., Suzuki, T., and Tomizawa, K.
Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to
Mitochondrial Disease.
Cell Rep., vol. 22, no. 2, 482 (2018) [pubmed]
Akaike, T., Ida, T., Wei, F.-Y., Nishida, M., Kumagai, Y., Alam, M. M.,
Ihara, H., Sawa, T., Matsunaga, T., Kasamatsu, S., Nishimura, A., Morita, M., Tomizawa, K., Nishimura, A., Watanabe, S., Inaba, K., Shima, H., Tanuma, N., Jung, M., Fujii, S., Watanabe, Y.,
Ohmuraya, M., Nagy, P., Feelisch, M., Fukuto, J. M., and Motohashi, H.
Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics.
Nat. Commun., vol. 8, no. 1, 1177 (2017) [pubmed]
Fakruddin, M., Wei, F.-Y., Emura, S., Matsuda, S., Yasukawa, T., Kang, D.,
and Tomizawa, K.
Cdk5rap1-mediated 2-methylthio-N6-isopentenyladenosine modification is absent from nuclear-derived RNA species.
Nucleic Acids Res., vol. 45, no. 20, pp. 11954–11961 (2017) [pubmed]
Takahashi, N., Wei, F.-Y., Watanabe, S., Hirayama, M., Ohuchi, Y., Fujimura,
A., Kaitsuka, T., Ishii, I., Sawa, T., Nakayama, H., Akaike, T., and Tomizawa, K.
Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion.
Nucleic Acids Res., vol. 45, no. 1, pp. 435–445 (2017) [pubmed]
Wu, Y., Wei, F.-Y., Kawarada, L., Suzuki, T., Araki, K., Komohara, Y.,
Fujimura, A., Kaitsuka, T., Takeya, M., Oike, Y., Suzuki, T., and Tomizawa, K.
Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS Genet., vol. 12, no. 9, e1006355 (2016) [pubmed]
Wei, F.-Y., Zhou, B., Suzuki, T., Miyata, K., Ujihara, Y., Horiguchi, H., Takahashi, N., Xie, P., Michiue, H., Fujimura, A.,
Kaitsuka, T., Matsui, H., Koga, Y., Mohri, S., Suzuki, T., Oike, Y., and Tomizawa, K.
Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans.
Cell Metab., vol. 21, no. 3, pp. 428–442 (2015) [pubmed]
Zhou, B., Wei, F.-Y., Kanai, N., Fujimura, A., Kaitsuka, T., and Tomizawa,
K.
Identification of a splicing variant that regulates type 2 diabetes risk factor CDKAL1 level by a coding-independent mechanism in human.
Hum. Mol. Genet., vol. 23, no. 17, pp. 4639–4650 (2014) [pubmed]
Xie, P., Wei, F.-Y., Hirata, S., Kaitsuka, T., Suzuki, T., Suzuki, T., and
Tomizawa, K.
Quantitative PCR measurement of tRNA 2-Methylthio modification for assessing type 2 diabetes risk.
Clin. Chem., vol. 59, no. 11, pp. 1604–1612 (2013) [pubmed]
Ueda, Y., Wei, F.-Y., Hide, T.-I., Michiue, H., Takayama, K., Kaitsuka, T.,
Nakamura, H., Makino, K., Kuratsu, J.-I., Futaki, S., and Tomizawa, K.
Induction of autophagic cell death of glioma-initiating cells by cell-penetrating d-isomer peptides consisting of Pas and the p53 C-terminus.
Biomaterials, vol. 33, no. 35, pp. 9061–9069 (2012) [pubmed]
Wei, F.-Y., Suzuki, T., Watanabe, S., Kimura, S., Kaitsuka, T., Fujimura, A., Matsui, H., Atta, M., Michiue, H., Fontecave, M.,
Yamagata, K., Suzuki, T., and Tomizawa, K.
Deficit of tRNALys modification by Cdkal1 causes the development of type 2 diabetes in mice.
J. Clin. Invest., vol. 121, no. 9, pp. 3598–3608 (2011) [pubmed]
Arragain, S., Handelman, S. K., Forouhar, F., Wei, F.-Y., Tomizawa, K., Hunt, J.
F., Douki, T., Fontecave, M., Mulliez, E., and Atta, M.
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
J. Biol. Chem., vol. 285, no. 37, pp. 28425–28433 (2010) [pubmed]
Wei, F.-Y.,
Nagashima, K., Ohshima, T., Saheki, Y., Lu, Y.-F., Matsushita, M., Yamada, Y., Mikoshiba, K., Seino, Y., Matsui, H., and Tomizawa, K.
Cdk5-dependent regulation of glucose-stimulated insulin secretion.
Nat. Med., vol. 11, no. 10, pp. 1104–1108 (2005) [pubmed]